rabbit anti-rat cd31 antibody  (Servicebio Inc)

Journal: bioRxiv

Article Title: Amyloid β induces cardiac dysfunction and neuro-signaling impairment in the heart of an Alzheimer’s disease model

doi: 10.1101/2023.07.11.548558

Figure Lengend Snippet: Top panels: Representative digital images (scale bar 50μm) showing endothelial cells (stained with CD31, in orange) and Aβ (in green). Bottom panels: representative digital images (scale bar 50μm) showing smooth muscle cells by α-Smooth muscle alpha-actin staining (αSMA, in red) and Aβ (in green) in cardiac sections from WT and Tg2576 mice. n=3-4 mice/group. WT mice are represented in the left panels and Tg2576 mice in the right panels.

Article Snippet: Via indirect immunofluorescence procedure, sections were analyzed using rabbit polyclonal antibodies against CD31 (AF3628-R&D Systems, 1:30) and α-smooth muscle actin (α-SMA, ab32575-Abcam, 1:500) at 4°C overnight, followed by specific secondary antibodies conjugated with cyanine 3 or cyanin 5 respectively, to stain vascular network.

Techniques: Staining

Journal: Regenerative Therapy

Article Title: A temperature-sensitive chitosan hydrogels loaded with nano-zinc oxide and exosomes from human umbilical vein endothelial cells accelerates wound healing

doi: 10.1016/j.reth.2025.04.020

Figure Lengend Snippet: IHC study for the expression of CD31 was performed to assess angiogenesis in the wounds. (A) Representative images of immunohistochemical staining of CD31 in wound beds at 14 days after surgery. Scale bar, 50 μm. (B) Quantitative analysis of the number of new blood vessel per field (n = 6). ∗∗∗ P < 0.001.

Article Snippet: Immunohistochemistry with a rabbit anti-rat CD31 antibody (Wuhan Servicebio Technology, China) was performed on tissue sections to assess angiogenesis in the wounds following intervention [ ].

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Plastic and Reconstructive Surgery

Article Title: Tracing the Change and Contribution of Subcutaneous Adipose to Skin Expansion Using a Luciferase-Transgenic Fat Transplantation Model

doi: 10.1097/PRS.0000000000010753

Figure Lengend Snippet: Subcutaneous adipose tissue promoted vascularization and cell proliferation in expanded skin by means of paracrine growth factors. ( Above ) Immunohistochemical staining for CD31 and PCNA to mark vessels and proliferating cells. ( Center ) mRNA expression levels of EGF, bFGF, and VEGF in skin samples from the control group and the AT group ( AT-Skin ), and adipose tissue from the AT group ( AT-Adipose ). ( Below , right ) Immunoblot analysis of the expression of bFGF, EGF, and VEGF in skin samples from the control group and the AT group, and adipose tissue from the AT group. Scale bar = 100 µm; * P < 0.05; *** P < 0.001.

Article Snippet: To detect the vessels and proliferating cells (early G 1 and S phases) in expanded skin, rabbit anti-rat CD31 (BM2966; Boster, Wuhan, People’s Republic of China) and mouse anti-rat proliferating cell nuclear antigen (PCNA) (BM0104; Boster) antibodies were applied.

Techniques: Immunohistochemical staining, Staining, Expressing, Control, Western Blot

Journal: Plastic and Reconstructive Surgery

Article Title: Tracing the Change and Contribution of Subcutaneous Adipose to Skin Expansion Using a Luciferase-Transgenic Fat Transplantation Model

doi: 10.1097/PRS.0000000000010753

Figure Lengend Snippet: Tracing of cells derived from adipose tissue. Cells derived from subcutaneous adipose tissue were labeled with an anti-luciferase antibody ( green ). ( Left ) Cells labeled with both luciferase and PDGFRα represent subcutaneous adipose tissue-derived stem cells, and ( center ) those labeled with both luciferase and DLK1 represent adipose tissue-derived preadipocytes. ( Right ) Cells derived from adipose tissue also differentiated into endothelial cells, as evidenced by Luc + /CD31 cells located in blood vessels. Scale bar = 100 µm.

Article Snippet: To detect the vessels and proliferating cells (early G 1 and S phases) in expanded skin, rabbit anti-rat CD31 (BM2966; Boster, Wuhan, People’s Republic of China) and mouse anti-rat proliferating cell nuclear antigen (PCNA) (BM0104; Boster) antibodies were applied.

Techniques: Derivative Assay, Labeling, Luciferase